Recombinant Potato leafroll virus Protein P1, partial

Product Specifications

Product Name Alternative

69.7 kDa protein; Genome-linked protein precursor; Protein ORF1

Abbreviation

Recombinant Potato leafroll virus protein P1 protein, partial

UniProt

P11622

Expression Region

400-639aa

Organism

Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)

Target Sequence

STAVKGRVFSDETVKELEREASEAVKKLARFKSLTGKNWANDYDSDEDYGLEKEAATNAPAEKTAQTNSAEKTAPSTSAEKTAPTNKPLNGRAAPPAKTNGNSDIPDAAISAPPMDKMVEQIITAMVGRINLSEIEEKIVSRVSQKALQKPKQNKRGRRGGKNKQNNLPPTSTQSISGAPKKKAVPQASGSAGISPATTTPAPKEKPSGGKNSAKFIPSWRIKQQDSAGQKPDLKLNSKA

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Precursor from which the VPg molecule is probably released at the onset of the RNA synthesis. Essential for virus replication.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

30.6 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.