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Uridine Diphosphate Glucose Prophosphorylase (UGP) Activity Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm.

Assay Performance Time

35 min

Sample Type

Animal tissue; plant tissue

Detection Range

0.5-70.0 U/L

Sensitivity

0.5 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

2.3-3.9%

Intra CV

1.0-3.2%

Detection Instrument

Microplate reader

Frequently Asked Questions

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