Recombinant Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT), partial
Product Specifications
Product Name Alternative
(V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)
Abbreviation
Recombinant Human TIGIT protein, partial
Gene Name
TIGIT
UniProt
Q495A1
Expression Region
22-141aa
Organism
Homo sapiens (Human)
Target Sequence
MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP
Tag
C-terminal 6xHis-Myc-tagged
Type
Developed Protein
Source
Mammalian cell
Field of Research
Immunology
Relevance
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
17.5 kDa
References & Citations
"The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells."Yu X., Harden K., Gonzalez L.C., Francesco M., Chiang E., Irving B., Tom I., Ivelja S., Refino C.J., Clark H., Eaton D., Grogan J.L.Nat. Immunol. 10:48-57 (2009)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12934660/
Protein Length
Partial
Available Sizes
Curated Selection
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