L17E

L17E is an attenuated cationic amphiphilic lytic (ACAL) peptide that can be used to deliver a variety of macromolecules, including proteins, antibodies, and DNA nanostructures. L17E inserts and cleaves the membrane structure through electrostatic interaction, enabling intracellular escape. The efficiency of L17E-mediated delivery is strongly correlated with the expression level of KCNN4 (the gene encoding the calcium-activated potassium channel KCa3.1) . L17E also promotes the cellular uptake of macromolecules by inducing micropinocytosis. L17E can be further optimized and improved through dimerization strategies and in combination with other delivery systems, such as nuclear localization signal peptides and cell membrane-coated nanoparticles[1][2][3][4][5][6].

Product Specifications

CAS Number

[1898254-09-5]

UNSPSC

12352209

Target

Potassium Channel

Type

Peptides

Related Pathways

Membrane Transporter/Ion Channel

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/l17e.html

Purity

99.66

Solubility

H2O : ≥ 100 mg/mL

Smiles

CC(C[C@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC1=CNC2=CC=CC=C12)NC([C@H]([C@H](CC)C)N)=O)=O)CC(C)C)=O)[C@@H](C)O)=O)C)=O)CC(C)C)=O)CCCCN)=O)CC3=CC=CC=C3)=O)CC(C)C)=O)=O)CCCCN)=O)CC4=CN=CN4)=O)C)=O)C)=O)CCCCN)=O)CC5=CN=CN5)=O)CCC(O)=O)=O)C)=O)CCCCN)=O)CCC(N)=O)=O)CCC(N)=O)=O)CC(C)C)=O)CO)=O)CCCCN)=O)C(N)=O)C

Molecular Formula

C134H220N38O31

Molecular Weight

2859.42

References & Citations

[1]Dogan Can Kirman, et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis. 2022 Feb 23;13 (2) :172.|[2]Nomura Y, et al. Improved cytosolic delivery of macromolecules through dimerization of attenuated lytic peptides. Bioorg Med Chem Lett. 2020 Sep 1;30 (17) :127362.|[3]Akishiba M, et al. Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide. Nat Chem. 2017 Aug;9 (8) :751-761.|[4]Du W, et al. Biodegradable silica nanocapsules enable efficient nuclear-targeted delivery of native proteins for cancer therapy. Biomaterials. 2023 Mar;294:122000. |[5]Kuriyama M, et al. KCNN4 as a genomic determinant of cytosolic delivery by the attenuated cationic lytic peptide L17E. Mol Ther. 2025 Feb 5;33 (2) :595-614.|[6]He, Jinjun, et al. Coating tetrahedral DNA framework with endosomolytic peptides for improved stability and cytosolic delivery. Advanced Sensor and Energy Materials 3.2 (2024) : 100098.