4-Nitrophenyl β-D-Cellobioside

4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog. 4-Nitrophenyl β-D-Cellobioside is hydrolyzed by β-glucosidases such as TxGH116 and ThCel7B. 4-Nitrophenyl β-D-Cellobioside can also be hydrolyzed by exoglucanases and endoglucanases to produce p-nitrophenol (PNP) . 4-Nitrophenyl β-D-Cellobioside can be used to detect cellulase activity[1][2][31][4][5].

Product Specifications

CAS Number

[3482-57-3]

Product Name Alternative

P-Nitrophenyl β-D-Cellobioside

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Glycosidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/4-nitrophenyl-β-d-cellobioside.html

Purity

99.94

Solubility

H2O : 5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)[C@@H]1OC3=CC=C([N+]([O-])=O)C=C3

Molecular Formula

C18H25NO13

Molecular Weight

463.39

Precautions

H302, H315, H319, H335

References & Citations

[1]Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371 (2) :146-53. |[2]Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21 (1) : 25-31.|[3]Charoenwattanasatien R, et al. Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2) . ACS Chem Biol. 2016 Jul 15;11 (7) :1891-900. |[4]Kudo K, et al. Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genome. J Biosci Bioeng. 2015 Dec;120 (6) :614-23.|[5]Pellegrini VO, et al. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Appl Microbiol Biotechnol. 2015 Nov;99 (22) :9591-604.