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Cy5 maleimide

Cy5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance[2].

Product Specifications

CAS Number

[1437796-65-0]

Product Name Alternative

Cyanine5 maleimide

UNSPSC

12171500

Hazard Statement

H315, H319

Target

DNA Stain; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/cy5-maleimide.html

Purity

99.43

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

O=C(NCCN(C1=O)C(C=C1)=O)CCCCCN(/C2=C/C=C/C=C/C(C3(C)C)=[N+](C4=CC=CC=C34)C)C5=CC=CC=C5C2(C)C.[Cl-]

Molecular Formula

C38H45ClN4O3

Molecular Weight

641.24

Precautions

H315, H319

References & Citations

[1]Maeda K, et al. Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms. Biochem J. 2004 Mar 1;378 (Pt 2) :497-507.|[2]Ptaszek M. Rational design of fluorophores for in vivo applications. Prog Mol Biol Transl Sci. 2013;113:59-108.|[3]Shindy, H. A. (2017) . Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029

Shipping Conditions

Blue Ice

Storage Conditions

-20°C (Powder, sealed storage, away from moisture and light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Available Sizes

Curated Selection

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