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H2AFX Antibody

This Phospho-Histone H2A.X (Ser139) Antibody is an unconjugated polyclonal product. It tagets H2AFX using a Peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X as the immunogen. This antibody is suitable for ELISA, IF, WB.

Product Specifications

Product Name Alternative

H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_HUMAN antibody; Histone H2A.X antibody

UniProt

P16104

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X.

Target

H2AFX

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Dilution

WB: 1:500-1:1000, IF: 1:100-1:200

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, IF, WB

Host or Source

Rabbit

Preservative

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Isotype

IgG

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