Anti-P95/NBS1 (Phospho-Ser343) Antibody

Rabbit Polyclonal Antibody specific to P95/NBS1 (Phospho-Ser343)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-P95/NBS1 (Phospho-Ser343) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of Serine 343 (S-L-S(p)-Q-G) derived from human p95/NBS1.

Target

P95/NBS1 (Phospho-Ser343)

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~95kDa is detected. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human p95/NBS1 only when phosphorylated at serine 343.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.