Anti-Grp170 Antibody

Mouse Monoclonal Antibody specific to Grp170

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170

Gene Name

HYOU1

NCBI Gene ID

UniProt

Q9Y4L1

Accession Number

NP_006389.3

Cellular Locus

Endoplasmic reticulum lumen.

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide sequence derived from human Grp170 (accession no. NP_006389.3)

Target Antigen

Hypoxia up-regulated protein 1

Target

Grp170

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ELISA

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {PubMed:10037731}.

Additionnal Information

ELISA: confirmed with antibody (1-10ug/ml) binding to Grp170 at 5ug/ml on the solid phase Immunoblotting: with antibody at 1.0-2.0ug/ml. A band of 170kDa is detected. These are recommended concentrations End user should determine optimal concentrations for their applications. Positive control: HEK293 cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human Grp170 (170kDa).

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Grp170 is a major chaperone/stress protein resident in the ER. It is distantly related in sequence to both hsp110 and hsp70 families, but represents the Grp170 stress protein family. Studies have suggested that Grp170 is an important element of the protein processing machinery of the ER. Unlike Hsp110 and Hsp70, Grp170 has two distinct chaperoning domains; a classic beta-sheet peptide binding domain, with some COOH- terminal sequence, and a COOH-terminal alpha-helical domain. Like other members of the Hsp70 superfamily, Grp170 can elicit anti-tumor immune responses most likely by chaperoning antigenic peptide and binding to receptor(s) on antigen-presenting cells (APC).