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HCoV-NL63 Nucleocapsid protein, His Tag

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 43.2 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

Q6Q1R8-1

Expression Region

Met 1 - His 377

Host

E. coli

Target

Nucleocapsid protein

Conjugation

Unconjugated

Tag

N-6xHis

Source

HCoV-NL63

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Powder

Buffer

40 mM PB, 500 mM NaCl, pH7.4

Additives

Trehalose

Molecular Weight

43.2 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*2

Host or Source

E. coli

Species

HCoV-NL63

Protein ID

Q6Q1R8-1

Preservative

Trehalose

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