Anti-AMIGO-1 Antibody

Mouse Monoclonal Antibody specific to AMIGO-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

AMIGO-1, Alivin-2

Gene Name

AMIGO1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=AMIGO1">AMIGO1</a>

UniProt

Q86WK6

Cellular Locus

Cell membrane, Perikaryon, Cell projection, dendrite, Cell projection, axon

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 554-574, the cytoplasmic C- terminus, of human AMIGO-1 (accession no.Q86WK6).

Target Antigen

Amphoterin-induced protein 1

Target

AMIGO-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. {UniProtKB:Q80ZD7, UniProtKB:Q80ZD8}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~60-80kDa depending on maturity/glycosylation. Positive control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat AMIGO-1. It does not cross- react with AMIGO-2 or AMIGO-3.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO-1, AMIGO-2 and AMIGO-3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, (also designated Alivin2) promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons.