Mouse ICOS / CD278 (C137S, C138S) Protein, His Tag

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.7 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9WVS0-1 (C137S, C138S)

Expression Region

Glu 21 - Leu 144

Host

HEK293

Target

ICOS

Conjugation

Unconjugated

Tag

C-10xHis

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human B7-H2 Protein, Fc Tag (Cat. No. B72-H5254) at 5 μg/mL (100 μL/well) can bind Mouse ICOS (C137S, C138S) Protein, His Tag (Cat. No. ICS-M52H8) with a linear range of 39-625 ng/mL (QC tested).