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Recombinant Rat Fibroblast Growth Factor 9

Product Specifications

Background

Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9.

Product Name Alternative

GAF, Glia-activating factor, HBGF-9

Gene ID

25444

UniProt

P36364

Target

LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS

Purification

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 1 EU/ug of rRtFGF-9-1 as determined by LAL method.

Bioactivity

6

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0.

Host or Source

Escherichia coli

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