Recombinant Human Platelet-derived Growth Factor-CC
Product Specifications
Target
Val235-Gly345, with an N-terminal Met and 6-His tag; Accession # NP_057289
Purification
> 97 % by SDS-PAGE analyses.
Endotoxin
Less than 0.1 EU/ug of rHuPDGF-CC as determined by LAL method.
Bioactivity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/ml.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
Formulation
Lyophilized from 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Host or Source
Escherichia coli
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