Calmodulin-tag Binding/ Wash Buffer

<strong>Calmodulin-tag Binding/ Wash Buffer </strong>_x000D_ <strong>Catalog number:</strong> B2016319_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 mL_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> RT_x000D_ <strong>Keywords:</strong> Calmodulin Binding/ Wash Buffer_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Li CQ, Ye P, Cao Zf, Wang H, Lu L, Nicastro P, Wood E, Robert JJ, Ouwehand WH, Hill F, López JA, Wardell MR. Expression of the amino-terminal domain of platelet glycoprotein Ib alpha: exploitation of a calmodulin tag for determination of its functional activity Protein Expr Purif. 2001 Jul;22(2):200-10._x000D_ 2: Chad JE, Eckert R. An enzymatic mechanism for calcium current inactivation in dialysed Helix neurones J Physiol. 1986 Sep;378:31-51._x000D_ 3: Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol. 2003 Jan;60(5):523-33._x000D_ 4: Murray G, Marshall MJ, Trumble W, Magnuson BA. Calmodulin-binding protein detection using a non-radiolabeled calmodulin fusion protein Biotechniques. 2001 May;30(5):1036-42._x000D_ 5: Lin L, Liu C, Nayak BC, He W, You M, Yuchi Z. A two-step purification strategy using calmodulin as an affinity tag J Chromatogr A. 2018 Apr 6;1544:16-22._x000D_ 6: Mukherjee S, Ura M, Hoey RJ, Kossiakoff AA. A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction J Mol Biol. 2015 Aug 14;427(16):2707-25._x000D_ 7: Zhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification J Anal Methods Chem. 2013;2013:581093._x000D_ 8: Klein W. Calmodulin-binding peptide as a removable affinity tag for protein purification Methods Mol Biol. 2003;205:79-97._x000D_ 9: Li Y. Commonly used tag combinations for tandem affinity purification Biotechnol Appl Biochem. 2010 Feb 15;55(2):73-83._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/18924111">10: Battesti A, Bouveret E. Improvement of bacterial two-hybrid vectors for detection of fusion proteins and transfer to pBAD-tandem affinity purification, calmodulin binding peptide, or 6-histidine tag vectors Proteomics. 2008 Nov;8(22):4768-71. </a>_x000D_ _x000D_ <strong>Products Related to Calmodulin-tag Binding/ Wash Buffer can be found at</strong> <a href="https://moleculardepot.com/product-category/Chemicals/"> Chemicals</a>