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Biotinylated SARS-CoV-2 Spike NTD Protein, His, Avitag™ (BA.2.86/Denmark/EPI_ISL_18114953) (MALS verified)

Biotinylated SARS-CoV-2 Spike NTD Protein, His, Avitag (BA.2.86/EPI_ISL_18114953) (SPD-C82H8) is expressed from human 293 cells (HEK293) . It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (ins16MPLF, T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.86/Denmark/EPI_ISL_18114953) .

Product Specifications

Background

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.2 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (ins16MPLF, T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D)

Expression Region

Ser 13 - Leu 303

Host

HEK293

Target

Spike NTD

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Spike NTD Protein, His,Avitag (BA.2.86/EPI_ISL_18114953) (Cat. No. SPD-C82H8) at 1 μg/mL (100 μL/well) on streptavidin precoated (STN-N5116) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) with a linear range of 0.1-1 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.2 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (ins16MPLF, T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D)

Preservative

Trehalose

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