Biotinylated Human latent GDF-8 Protein, His, Avitag™ (pro & latent)

Growth differentiation factor 8 (GDF8), also known as myostatin, is a unique member of the transforming growth factor-β superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions. GDF8 acts as a negative regulator of skeletal muscle growth and differentiation. In addition to the expression in the musculoskeletal system, GDF8 is also expressed in various tissues, including the reproductive system.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 43.7 kDa. The protein migrates as 40 kDa and 53-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

O14793-1

Expression Region

Asn 24 - Ser 375

Host

HEK293

Target

GDF-8

Conjugation

Biotin-labeled

Tag

N-Avi & N-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent GDF-8 Protein, His,Avitag (Cat. No. GD8-H82Q3) with a linear range of 0.5-31 ng/mL (QC tested).