Recombinant Dermatophagoides farinae Mite allergen Der f 6 (DERF6)

Product Specifications

Product Name Alternative

(Allergen Der f VI) (DF5) (allergen Der f 6)

Abbreviation

Recombinant Dermatophagoides farinae DERF6 protein

Gene Name

DERF6

UniProt

P49276

Expression Region

50-279aa

Organism

Dermatophagoides farinae (American house dust mite)

Target Sequence

VVGGQDADLAEAPFQISLLKDYLIMKRHMCGGSLISESTVVTAAHCTYGQKASSLSVRYGTNQRTSSSYGDLKVKPIIQHESYEQDQTQTDKTIIILPNPVVPSTNVQMNEIETEDIVDGDKVTIYGWGLTDGNGKDLPDKLQKGSMTIVGNDRCNEKWGSINAIHPGMICALDKTQSGCNGDSGGPLVSANRKLTGIVSWGPSKCPPGEYMSVFTRPKYYLDWITKNIV

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Protease that shows specificity similar to chymotrypsin.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

32.5 kDa

References & Citations

"Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae." Kawamoto S., Mizuguchi Y., Morimoto K., Aki T., Shigeta S., Yasueda H., Wada T., Suzuki O., Jyo T., Ono K. Biochim. Biophys. Acta 1454:201-207 (1999)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.