CheKine™ Micro Acetokinase (ACK) Activity Assay Kit

Acetokinase (ACK) is primarily found in microorganisms and functions as a key enzyme in bacterial carbon metabolism and energy metabolism, playing a central role particularly in the methanogenesis pathway of archaea. ACK catalyzes the conversion of acetate and ATP into acetyl phosphate and ADP. Subsequently, pyruvate kinase catalyzes the formation of ATP and pyruvate from ADP and PEP. Lactate dehydrogenase then catalyzes the reaction that converts pyruvate and NADH into lactate and NAD+. By measuring the rate of NADH oxidation to NAD+ at a wavelength of 340 nm, one can determine the activity of ACK.

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Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.