Recombinant Xenopus tropicalis Hyaluronan synthase 1 (has1), partial

Product Specifications

Product Name Alternative

(Hyaluronate synthase 1) (Hyaluronic acid synthase 1) (HA synthase 1)

Abbreviation

Recombinant Xenopus tropicalis has1 protein, partial

Gene Name

Has1

UniProt

B1WB39

Expression Region

82-401aa

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Target Sequence

AYLEIRRINKTDLPCSFKKTVALTIAGYQENPDYLKHCLDSCRYVKYPKDKLKIILVIDGNTEDDAYMMEMFKDVFHGDDVGTYVWKGNYHTGVKETQDGSCPEVSKPLNEDEGIRIVEELVRTKRCVCIMQQWGGKREVMYTAFRAIGTTMDYVQVCDSDTKLDELATVEMVKVLEANELCGAVGGDVRILNPYDSFISFMSSLRYWMAFNVERACQSYFDCVSCISGPLGMYRNDILQVFLEAWHSQKFLGTYCTLGDDRHLTNRVLSMGYRTKYTPKCRAFSETPSQYLRWLNQQTRWTKSYFREWLYNAQWWYKHH

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

44.9 kDa

References & Citations

NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.