Chicken anti Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)
Product Specifications
UniProt
P0DPI0
Host
Chicken
Type
Primary Antibodies
Source
Chickens were immunized with highly purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain) (HcA) (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type A Heavy Chain Binding Domain was immunoaffinity purified using the immunogen immobilized on a solid support.
Applications
WB, ELISA
Field of Research
Infectious Agents and Disease, Microbiology, Toxic Compounds
Purification Method
Egg-yolk derived IgY Affinity Purified
Assay Principle
This antibody is suitable for ELISA and Western Blotting; For direct ELISA, a 1:10,000 dilution detects 2 µg coating of Botulinum Toxin A. For Western blot, the recommended dilution to probe 0.5 µg of unreduced holotoxin or heavy chain is 1:2000.
Concentration
See vial for Concentration
Form
Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Shipping Conditions
Ambient Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
CAS Number
9007-83-4
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