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Chicken anti Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)

Product Specifications

CAS Number

9007-83-4

UniProt

P0DPI0

Host

Chicken

Type

Primary Antibodies

Source

Chickens were immunized with highly purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain) (HcA) (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type A Heavy Chain Binding Domain was immunoaffinity purified using the immunogen immobilized on a solid support.

Applications

WB, ELISA

Field of Research

Infectious Agents and Disease, Microbiology, Toxic Compounds

Purification Method

Egg-yolk derived IgY Affinity Purified

Assay Principle

This antibody is suitable for ELISA and Western Blotting; For direct ELISA, a 1:10,000 dilution detects 2 µg coating of Botulinum Toxin A. For Western blot, the recommended dilution to probe 0.5 µg of unreduced holotoxin or heavy chain is 1:2000.

Concentration

See vial for Concentration

Form

Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

Shipping Conditions

Ambient Temperature

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

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