Chicken anti Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)

Product Specifications

CAS Number

9007-83-4

UniProt

P0DPI0

Host

Chicken

Type

Primary Antibodies

Source

Chickens were immunized with highly purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain) (HcA) (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type A Heavy Chain Binding Domain was immunoaffinity purified using the immunogen immobilized on a solid support.

Applications

WB, ELISA

Field of Research

Infectious Agents and Disease, Microbiology, Toxic Compounds

Purification Method

Egg-yolk derived IgY Affinity Purified

Assay Principle

This antibody is suitable for ELISA and Western Blotting; For direct ELISA, a 1:10,000 dilution detects 2 µg coating of Botulinum Toxin A. For Western blot, the recommended dilution to probe 0.5 µg of unreduced holotoxin or heavy chain is 1:2000.

Concentration

See vial for Concentration

Form

Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.