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Anti-Rat IgG H&L Chain Antibody, Alkaline Phosphatase conjugate

Goat Polyclonal Antibody specific to Rat IgG H&L Chain-Alk Phos

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Rat IgG H&L Chain-Alk Phos Goat Polyclonal Antibody

Host

Goat

Reactivity

Rat

Immunogen

Rat IgG

Target

Rat IgG H&L Chain

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Secondary Antibodies

Purification Method

Purified by affinity chromatography on rat IgG H&L

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

HEPES

Additionnal Information

Immunoblotting: use at a dilution of 1:5,000-1:50,000. <br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000 <br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000 <br><br>ELISA: use at a dilution of 1:5,000-1:50,000. <br><br>These are recommended dilutions. <br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.

Storage Conditions

This antibody is stable for at least 1 year at 2-8°C.

Specificity

Antibody is affinity-purified on rat IgG and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG of other species.

Formulation

50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3

Buffer pH

pH 7.1

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