SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C5221) is expressed from human 293 cells (HEK293) .

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

YP_009724397.2

Host

HEK293

Target

Nucleocapsid protein

Conjugation

Unconjugated

Tag

C-6xHis

Source

SARS-CoV-2

Stability

● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.1-3 ng/mL (QC tested).

Format

Liquid

Buffer

10 mM PB, 150 mM NaCl, pH7.4

Molecular Weight

47.3 kDa

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

5mg*2

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/NUN-C5221.pdf

Species