Demecolcine

Demecolcine is a potent mitotic inhibitor with an IC50 value of 2.4 μM for inhibition of tubulin polymerization. Colcemid (Demecolcine) can interact with tubulin dimers to induce anti-mitotic action and inhibit microtubule growth. Colcemid (Demecolcine) can be used for inflammatory disorders and cancer research[1][2].

Product Specifications

CAS Number

[477-30-5]

UNSPSC

12352005

Hazard Statement

H300

Target

Apoptosis; Microtubule/Tubulin

Type

Natural Products

Related Pathways

Apoptosis; Cell Cycle/DNA Damage; Cytoskeleton

Applications

COVID-19-anti-virus

Field of Research

Cancer; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/colcemid.html

Purity

99.91

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C1C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=CC=C1OC

Molecular Formula

C21H25NO5

Molecular Weight

371.43

Precautions

H300

References & Citations

[1]T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31 (4) :295-9.|[2]Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv.|[3]Nett RS, et al. Discovery and engineering of colchicine alkaloid biosynthesis. Nature. 2020 Aug;584 (7819) :148-153. doi: 10.1038/s41586-020-2546-8. Epub 2020 Jul 22. Erratum in: Nature. 2020 Jul 30.|[4] Muzaffar A, et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J Med Chem. 1990 Feb;33 (2) :567-71.|[5]Risley MS, et al. An improved method for cytogenetic analysis of meiotic aneuploidy in rodent and frog spermatocytes. Mutat Res. 1990 Dec;234 (6) :361-8.