Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Library Pack

Selection of Mouse Monoclonal antibodies specific to Elastase (Neutrophil)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies: 13201 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA11-207.2 - Isotype IgM 13202 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA11-207.3 - Isotype IgM 13203 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA10-101.5 - Isotype IgG1 13204 - Anti-Elastase (Neutrophil) Mouse Monoclonal Antibody - Clone ELA10-103.2 - Isotype IgG2b

Gene Name

ELANE

Gene ID

130130

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=ELANE">ELANE</a>

UniProt

P08246

Cellular Locus

Cytoplasmic vesicle, phagosome

Host

Mouse

Reactivity

Human

Immunogen

Purified human neutrophil elastase.

Target Antigen

Neutrophil elastase

Target

Elastase (Neutrophil)

Clonality

Monoclonal

Type

Antibody Library Pack

Applications

ELISA

Field of Research

Enzymes

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

PBS

Function

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}.

Additionnal Information

These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

These antibodies recognize human neutrophil elastase and vary in their ability to inhibit neutrophil elastase activity as determined in an assay of elastase-mediated hydrolysis of elastin-Congo Red substrate:

Formulation

PBS, pH 7.4

Buffer pH

7.4

Target Background

Neutrophil elastase is a major neutral protease present in human neutrophils and is released by neutrophils following stimulation with a variety of stimuli. It is believed to be a major mediator of the lung destruction seen in emphysema. In addition to elastin, neutrophil elastase can cleave other biologically active proteins such as fibrinogen and antithrombin and is released by neutrophils during clot formation.