Anti-Glypican-1 Antibody

Mouse Monoclonal Antibody specific to Glypican-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Glypican-1 Mouse Monoclonal Antibody

Gene Name

GPC1

NCBI Gene ID

UniProt

P35052

Cellular Locus

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.; [Secreted glypican-1]: Secreted, extracellular space.

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Recombinant full-length human glypican-1.

Target Antigen

Glypican-1 [Cleaved into: Secreted glypican-1]

Target

Glypican-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IF

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. {PubMed:19936054, PubMed:21642435}.

Additionnal Information

ELISA: at 1-5ug/ml will detect recombinant glypican-1 on the solid phase at 200ng/well. Immunoblotting: at 1-10ug/ml, a band of approx. 64 kDa is detected in normal mouse brain. Immunofluorescence: at 1-10ug/ml, positive staining of human pancreatic carcinoma cell line Capan-1. These are recommended concentrations. User should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes native and recombinant human and mouse glypican-1.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Glypican-1 is a widely expressed, GPI anchored, cell surface proteoglycan that has been reported to be important in cellular adhesion and migration. Glypican-1 also functions as an accessory molecule for signaling receptors. Glypican-1 is overexpressed in human gliomas and pancreatic ductal cell carcinomas. Expression of glypican-1 is associated with the aggressive behavior of tumor cells in vitro and in vivo.