Human IL-35 Protein, Fc Tag
Human IL-35, Fc Tag (IL5-H5259) is expressed from human 293 cells (HEK293) . It contains AA Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha) (Accession # Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha) ) .
Product Specifications
Background
Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression. IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation. The heterodimeric cytokine of IL-35 involves two subunits of p35 and Ebi3 which are also seen in IL-12 and IL-27. IL-35 is recognized as a defi nite immunosuppressor with a huge potent of suppression.
Specifications
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.3 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)
Expression Region
Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha)
Host
HEK293
Target
IL-35
Conjugation
Unconjugated
Tag
C-Fc
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human IL-35 R, His Tag (Cat. No. ILR-H52H8) at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag (Cat. No. IL5-H5259) with a linear range of 0.002-0.625 μg/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
73.3 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/IL5-H5259.pdf
Species
Human
Curated Selection
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