Human IL-35 Protein, Fc Tag

Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression. IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation. The heterodimeric cytokine of IL-35 involves two subunits of p35 and Ebi3 which are also seen in IL-12 and IL-27. IL-35 is recognized as a defi nite immunosuppressor with a huge potent of suppression.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.3 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)

Expression Region

Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha)

Host

HEK293

Target

IL-35

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-35 R, His Tag (Cat. No. ILR-H52H8) at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag (Cat. No. IL5-H5259) with a linear range of 0.002-0.625 μg/mL (QC tested).