Anti-Versican Antibody

Mouse Monoclonal Antibody specific to Versican

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Large fibroblast proteoglycan, PG-M

Gene Name

Vcan

NCBI Gene ID

UniProt

Q62059

Cellular Locus

Secreted, extracellular space, extracellular matrix, Cell projection, cilium, photoreceptor outer segment, Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 362-585 of mouse Versican (accession no. Q62059).

Target Antigen

Versican core protein

Target

Versican

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is >350kDa. <br><br>Positive control: Mouse brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat Versican.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteo- glycan involved in cell growth and differentiation. Versican contains hyaluronic acid and glycosaminoglycan- binding domains,epidermal growth factor- like repeats, a Lectin-like sequence, and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in atherosclerotic lesions suggests a role for Versican in atherogenesis.