Bromocresol green

Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form) . Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells[1][2][3][4].

Product Specifications

CAS Number

[76-60-8]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/bromocresol-green.html

Concentration

10mM

Purity

99.59

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2C)(C3=CC(Br)=C(O)C(Br)=C3C)C4=CC=CC=C41)=O

Molecular Formula

C21H14Br4O5S

Molecular Weight

698.01

Precautions

H302, H315, H319, H335

References & Citations

[1]Delanghe S, et al. Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. Clin Chem Lab Med. 2018 Feb 23;56 (3) :436-440. |[2]Jurmanović S, et al. Organically modified silicate thin films doped with colourimetric pH indicators methyl red and bromocresol green as pH responsive sol–gel hybrid materials[J]. Thin Solid Films, 2010, 518 (8) : 2234-2240. |[3]Chaiyo S, et al. A novel paper-based colorimetry device for the determination of the albumin to creatinine ratio. Analyst. 2018 Nov 5;143 (22) :5453-5460. |[4]Hou H, et al. Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells. Sci Rep. 2017 May 11;7 (1) :1759.