Human CD40 Ligand / TNFSF5 Protein, Fc Tag (N297A), active trimer, premium grade

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 74.5 kDa. The protein migrates as 81 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P29965-1

Expression Region

Gly 116 - Leu 261

Host

HEK293

Target

CD40 Ligand

Conjugation

Unconjugated

Tag

N-Fc

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD40, His Tag (Cat. No. CD0-H5228) at 1 μg/mL (100 μL/well) can bind Human CD40 Ligand Protein, Fc Tag (N297A), premium grade (Cat. No. CDL-H5264) with a linear range of 0.02-3 ng/mL (QC tested).