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Anti-Hu CD368 APC

CD368 is an approximately 25-30 kDa C-type lectin, which serves as one of pattern recognition receptors of the innate immune system. It recognizes pathogen-associated molecular patterns of bacteria and fungi, such as alpha-mannans or trehalose 6,6´-dimycolate, and transfers the signal downstream using associated Fc receptor gamma chain. CD368 is expressed on neutrophils, monocytes, and on some populations of blood dendritic cells, but it decreases during differentiation of monocytes into dendritic cells or macrophages. Increased expression can be induced by IFN-gamma, TNF-alpha, IL-6, and IL-10. CD368 triggering leads to driving of antigen-presenting cells maturation and to promotion of T cell differentiation into Th1 and Th17.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

CD368 ectodomain fused with human Fc

Target Antigen

CD368

Clone

9B9

Applications

FC (QC tested)

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5 (9) :e100., URL: https://pubmed.ncbi.nlm.nih.gov/27766148/, *Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4 (11) :e50. , URL: https://pubmed.ncbi.nlm.nih.gov/26682057/, *Clark G, Stockinger H, Balderas R, van Zelm MC, Zola H, Hart D, Engel P: Nomenclature of CD molecules from the Tenth human leucocyte differentiation antigen workshop. Clin Transl Immunology 2016 Jan 22;5 (1) :e57., URL: https://pubmed.ncbi.nlm.nih.gov/26900471/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 9B9 recognizes an extracellular epitope of CD368, a type II transmembrane protein of C lectin family, expressed mainly on monocytes and neutrophils.

Applications Notes

6

Isotype

Mouse IgG2b kappa

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