Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer Protein, His, Avitag™ (MALS verified)

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293) . It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1) . Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 147.2 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q0ZME7-1

Expression Region

Ala 13 - Asn 1276

Host

HEK293

Target

Spike protein

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

HCoV-HKU1

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.