Anti-Heme Oxygenase-1 Antibody

Mouse Monoclonal Antibody specific to Heme Oxygenase-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HO-1, EC 1.14.14.18, HSP32

Gene Name

Hmox1

NCBI Gene ID

UniProt

P06762

Accession Number

NP_036712.1

Cellular Locus

Microsome. Endoplasmic reticulum membrane, Peripheral membrane protein; Cytoplasmic side.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

His-tagged rat heme-oxygenase-1 (HO-1)

Target Antigen

Heme oxygenase 1

Target

Heme Oxygenase-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Enzymes

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Additionnal Information

ELISA: confirmed with antibody at 1-10ug/ml binding to 5ug/ml HO-1 on the solid phase. Immunoblotting: confirmed with antibody at 1.0-2.0ug/ml and 20ug of rat kidney lysate. A band of ~31kDa is detected. Positive control: Rat kidney lysate. These are recommended concentrations; Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat HO-1. It does not cross-react with HO-2.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.