Recombinant Campylobacter fetus S-layer protein (sapA), partial
Product Specifications
Product Name Alternative
Surface array protein
Abbreviation
Recombinant Campylobacter fetus sapA protein, partial
Gene Name
SapA
UniProt
P35827
Expression Region
1-268aa
Organism
Campylobacter fetus
Target Sequence
MLNKTDVSMLYITIMGMASEGDGNKYWLDYANNNSLGVSSLANIMLDSPGAAKFFGDSLLAGNEKDFVTKIYSIALGNTSDVDGINYWTKAITGGGEFTDSKGNVISVASLSKGDLIGAMINSMVNGGSAESKAIFEAKAAASDYFADATLVRDISGLDEGTTSKLISEINSASDLDKVKSEIDALKSELPNPGSTYDLTEGNDNLKGTDLDDTFNGTTYVGNGTNKSTLSAFDKIDGGAGRDTLNAIFTANNNAAAATKLDQAEIDK
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
The S-layer is a paracrystalline mono-layered assembly of proteins which coats the surface of bacteria. This protein is critical for virulence.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
33.0 kDa
References & Citations
"Surface array protein of Campylobacter fetus. Cloning and gene structure." Blaser M.J., Gotschlich E.C. J. Biol. Chem. 265:14529-14535 (1990)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
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