Mouse IgG2a FITC Negative Control

Product Specifications

Background

Negative control for immunofluorescence staining with FITC conjugated mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to Human cell surface antigens.

Host

Mouse

Isotype

IgG2a

Type

Single-Color Negative Control

Applications

Flow Cytometry

Field of Research

CD markers, Flow Cytometry, Immunology

Purification Method

Protein A/G Chromatography

Assay Principle

Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood._x000D_ _x000D_ PBMC: Add10 μl of MAB/10^6 PBMC in 100 μl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. _x000D_ _x000D_ WHOLE BLOOD: Add10 μl of MAB/100 μl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

Shipping Conditions

Room Temperature

Storage Conditions

Product should be stored at 4-8ºC; DO NOT FREEZE. Store protected from light. Reagent is stable for the period shown on the vial when stored properly.