Cynomolgus Coagulation Factor III / Tissue Factor Protein, His Tag (active enzyme, MALS verified)

Full-length tissue factor (TF) is a transmembrane receptor and cofactor for factor (F) VII/FVIIa. In addition to full-length TF, an alternative spliced (as) form of TF can be generated that lacks the transmembrane domain and is released fromcells. In contrast to TF, asTF has low procoagulant activity because it lacks the transmembrane domain. Tissue factor is expressed by cells around blood vessels, such as adventitial fibroblasts, and body surfaces, such as epithelial cells, and plays a critical role in hemostasis. TF also contributes to various forms of thrombosis. Many cancers, particularly adenocarcinomas, express high levels of TF. A high level of tumor TF expression is associated with poor prognosis in many types of cancers, including breast, prostate, colorectal, and pancreatic cancer.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A2K5VX02-1

Expression Region

Ser 33 - Glu 252

Host

HEK293

Target

Coagulation Factor III

Conjugation

Unconjugated

Tag

C-10xHis

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Cynomolgus Tissue Factor, His Tag (Cat. No. TF3-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-TF Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).