mCherry Mouse Monoclonal Antibody [A9F2]
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. Genome editing has been improved greatly through the precise insertion of these fluorescent protein tags into the genetic material of many diverse organisms. Most comparisons between the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that affect protein performance in cells or organisms. It is hard to perfectly simulate cellular environments in vitro, and the difference in environment could have an effect on the brightness and photostability.
Product Specifications
CAS Number
9007-83-4
Swiss Prot
X5DSL3 AnaplasmaMarginale
Host
Mouse
Species Reactivity
Universal
Immunogen
Recombinant protein within mCherry aa 1-236 / 236.
Isotype
IgG2b
Clone
A9F2
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell
Concentration
1.17 mg/mL
Dilution
WB: 1:2,000-1:5,000 ;IF-Cell: 1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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