Anti-Myc (phospho-Thr58) Antibody

Rabbit Polyclonal Antibody specific to Myc (phospho-Thr58)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Myc (phospho-Thr58) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from human Myc and conjugated to KLH.

Target

Myc (phospho-Thr58)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~60kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat Myc only when phosphorylated at threonine 58.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Myc (c-Myc) is a regulator gene that codes for transcription factor Myc. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. A mutated version of Myc is found in many cancers which causes Myc to be constitutively expressed.