Mouse anti CD14

CD14 is a GPI-anchored molecule expressed by virtually all Human monocytes and macrophages and – to a lesser degree - granulocytes. CD14 together with Toll-like receptor 4 and MD-2 forms the LPS-receptor complex that recognizes and signals the presence of LPS. While CD14 has no signaling structure its main role seems to be the binding of LPS. The MEM18 antibody permits the identification and enumeration of leukocytes using flow cytometry. MEM18 has been also used for functional studies since this antibody blocks the interaction of LPS with CD14 on monocytes. _x000D_ Results must be interpreted by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us

Indirect Immunofluorescence (Staining Procedure) - Mix 20 µL Nordic-MUbio purified antibody with 50 µL whole blood or MNC suspension - Incubate for 15 minutes at 2-8°C - Wash cells with phosphate buffered saline (PBS) - Add to cell pellet 20 µL of affinity purified, fluorochrome labeled F(ab’)2 anti mouse Ig antibodies - Incubate for 15 minutes at 2-8°C - Wash cells with phosphate buffered saline (P and analyse by flow cytometry.

For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.

1. Beutler, B. (2002) Curr Top Microbiol Immunol 270, 109-20. _x000D_ 2. Goyert, S. M. (1989) In Leukocyte Typing IV (Oxford University Press, Oxford-New York-Tokyo) p789-793_x000D_ 3. Goyert, S. M., Ferrero, E., Rettig, W. J., Yenamandra, A. K., Obata, F. , Le Beau, M. M. (1988) Science 239, 497-500. _x000D_ 4. Goyert, S. M., Ferrero, E. M., Seremetis, S. V., Winchester, R. J., Silver, J. , Mattison, A. C. (1986) J Immunol 137, 3909-14. _x000D_ 5. Juan, T. S., Hailman, E., Kelley, M. J., Busse, L. A., Davy, E., Empig, C. J., Narhi, L. O., Wright S. D. , Lichenstein, H. S. (1995) J Biol Chem 270, 5219-24. _x000D_ 6. Knapp, W. (1989) In Leukocyte typing IV (Oxford University Press, Oxford-New York-Tokyo) p747-780 _x000D_ 7. Means, T. K., Lien, E., Yoshimura, A., Wang, S., Golenbock, D. T. , Fenton, M. J. (1999) J Immunol 163, 6748-55. _x000D_ 8. Zilberman, M., Goyert, S. M. , Vogel, S. N. (2001) J Immunol 166, 574-81. _x000D_ 9. Tapping, R. I., Akashi, S., Miyake, K., Godowski, P. J. , Tobias, P. S. (2000) J Immunol 165, 5780-7. _x000D_ 10. Ugolini, V., Nunez, G., Smith, R. G., Stastny, P. , Capra, J. D. (1980) Proc Natl Acad Sci U S A 77, 6764-8. _x000D_ 11. Yoshimura, A., Lien, E., Ingalls, R. R., Tuomanen, E., Dziarski, R. , Golenbock, D. (1999) J Immunol 163, 1-5.

Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody; For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.