Human Alpha-Synuclein (A53T) Protein, His Tag (MALS verified)

Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD) . Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.4 kDa. The protein migrates as 18-19 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

P37840-1 (A53T)

Expression Region

Met 1 - Ala 140

Host

E. coli

Target

Alpha-Synuclein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Alpha-Synuclein (A53T), His Tag (Cat. No. ALN-H51H5) at 1 μg/mL (100 μL/well) can bind Synuclein alpha/beta Monoclonal Antibody with a linear range of 1-31 ng/mL (QC tested).