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Monkeypox virus (strain Zaire-96-I-16) A29L Protein, His Tag

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. A29L binds to cell surface heparin to promote fusion of viral membrane with host plasma membrane.

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. A29L binds to cell surface heparin to promote fusion of viral membrane with host plasma membrane.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.4 kDa. The protein migrates as 16 kDa,18-20 kDa and 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q77HM6

Expression Region

Met 1 - Glu 110

Host

HEK293

Target

A29L (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (strain Zaire-96-I-16)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (strain Zaire-96-I-16) A29L Protein, His Tag (Cat. No. A2L-M52H3) at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox-A29L-Antibody with a linear range of 0.1-13 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

14.4 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*2

Host or Source

HEK293

Species

Monkeypox virus (strain Zaire-96-I-16)

Protein ID

Q77HM6

Preservative

Trehalose

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