Glucagon ELISA Kit

Glucagon is a peptide hormone encoded by the GCG gene and is produced by alpha cells in the islets of langerhans of pancreas. It plays an important role in carbohydrate metabolism. When the glucose levels are low in the bloodstream, glucagon elevates the glucose level by promoting gluconeogenesis and glycogenolysis, which is in contrast to Insulin. However, glucagon and insulin work together to keep the blood glucose level stable. Abnormal glucagon levels are found in some pathological conditions such as glucagonoma, a rare tumor of the pancreatic alpha cells that results in excessive production of glucagon associated with mild diabetes, weight loss etc. Some diabetic patients have low glucagon levels too. Therefore, measuring glucagon levels are crucial for treatment and diagnostic purposes. BioVision’s Glucagon ELISA Kit is a competitive ELISA kit for the quantitative determination of glucagon concentration in various biological samples such as serum and plasma. In this assay, glucagon-BSA conjugate is pre-coated on the 96-well plate. Glucagon in the sample and the antigen on the plate compete for binding to the anti-glucagon antibody. After the addition of the HRP conjugate that binds to captured glucagon antibody, TMB substrate is added and a color signal is developed. The intensity of color is inversely proportional to the amount of glucagon in the samples. This value is then compared to the glucagon standard curve and the glucagon concentration in sample(s) is calculated. The kit can detect as little as 10 ng/ml glucagon in less than 3 hr.