Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein (Monomer, MALS verified)

Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC) . Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC) . Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. The Human HLA-A*2402 EBV EBNA3A (RYSIFFDYM) complex protein is a complex of HLA-A*2402 of the MHC Class I, B2M and RYSIFFDYM peptide of the EBV EBNA3A.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV EBNA3A peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.8 kDa and 14.0 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & RYSIFFDYM

Expression Region

Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & RYSIFFDYM peptide

Host

HEK293

Target

HLA-A*2402 & B2M & EBV EBNA3A (RYSIFFDYM)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein (Cat. No. HLA-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.2-2 ng/mL (QC tested).