Human IL-1α Protein, His tag (Animal-Free)

Interleukin-1 alpha (IL1 alpha or IL1α) is a member of the interleukin-1 cytokine family, found constitutively present in epithelial layers of the entire gastrointestinal tract, lung, liver, kidney, endothelial cells, and astrocytes. The synthesized IL-1 alpha is a 31 kDa inactive precursor and can be cleaved by intracellular caspase-1 or extracellular proteases to generate the bioactive 17 kDa form and the 16 kDa N-terminal cleavage product. Both precursor and mature IL-1 alpha protein bind to the IL-1 receptor (IL-1R), initiating a cascade of inflammatory cytokines and chemokines production such as IL-6, IL-8, and TNF, in response to viral and bacterial pathogens conditions. IL-1 alpha plays a central role in immune-surveillance mechanisms, stimulating macrophages, neutrophils, and CD8+ T cells activity.

Product Specifications

Background

Product Name Alternative

Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte

Gene ID

3552

Accession Number

P01583

Tag

IL-1α

Type

Cytokines and proteins

Applications

His tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <10 pg/mL.The specific activity of recombinant human IL-1 alpha is approximately >1 x10⁸ IU/ mg.

Molecular Formula

19 kDa

Molecular Weight

19 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human IL-1α Protein consists of 159 amino acids and predicts a molecular mass of 19 kDa.

Formulation

Lyophilized from sterile PBS, pH 8.0.

Applications Notes

Human

Other Product Names

IL-1α, IL 1α, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.