Human ACE2 / ACEH Protein, His Tag (MALS verified)
Human ACE2, His Tag (AC2-H52H8) is expressed from human 293 cells (HEK293) . It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1) .
Product Specifications
Background
Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 85.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
Q9BYF1-1
Expression Region
Gln 18 - Ser 740
Host
HEK293
Target
ACE2
Conjugation
Unconjugated
Tag
N-10xHis
Source
Human
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.02-0.156 μg/mL (QC tested).
Format
Liquid
Buffer
50 mM Tris, 150 mM NaCl, Arginine, pH7.5
Additives
Glycerol
Molecular Weight
85.5 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/AC2-H52H8.pdf
Species
Human
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