Human Proprotein Convertase 9, PCSK9 ELISA Kit
Product Specifications
Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Product Name Alternative
Proprotein convertase subtilisin/kexin type 9
Synonyms
NARC1; FH3; HCHOLA3; LDLCQ1; PC9
Gene Name
PCSK9
Gene ID
255738
UniProt
Q8NBP7
Target
PCSK9
Field of Research
Signal transduction, Cardiovascular, Cell biology, Metabolism, Neuroscience, Stem cells
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 30000 pg/ml | 3.023 | 3.148 | 3.039 3.272 15000 pg/ml | 1.624 | 1.649 | 1.54 1.673 7500 pg/ml | 0.782 | 0.813 | 0.704 0.843 3750 pg/ml | 0.445 | 0.464 | 0.355 0.482 1875 pg/ml | 0.26 | 0.272 | 0.163 0.283 937.5 pg/ml | 0.169 | 0.181 | 0.072 0.193 468.75 pg/ml | 0.14 | 0.138 | 0.029 0.136 0 pg/ml | 0.112 | 0.109 | 0 0.105
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
468.75-30000 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 2674 | 134 | 5 Intra-assay | 2 | 20 | 5690 | 221 | 3.9 Intra-assay | 3 | 20 | 17781 | 808 | 4.5 Inter-assay | 1 | 20 | 2692 | 266 | 9.9 Inter-assay | 2 | 20 | 5707 | 569 | 10 Inter-assay | 3 | 20 | 17915 | 1501 | 8.4
Sensitivity
206.5 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
74286
Available Sizes
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