Human Galectin-1, LGALS1 ELISA Kit

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.

Concentration | O.D. | Average | Corrected ------------------------------------------ 20000 pg/ml | 2.418 | 2.463 | 2.381 2.507 10000 pg/ml | 1.3 | 1.316 | 1.234 1.331 5000 pg/ml | 0.743 | 0.706 | 0.624 0.668 2500 pg/ml | 0.346 | 0.371 | 0.289 0.395 1250 pg/ml | 0.21 | 0.224 | 0.142 0.237 625 pg/ml | 0.144 | 0.147 | 0.065 0.15 312.5 pg/ml | 0.103 | 0.108 | 0.026 0.112 0 pg/ml | 0.084 | 0.082 | 0 0.079

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1884 | 146 | 7.7 Intra-assay | 2 | 20 | 4095 | 223 | 5.4 Intra-assay | 3 | 20 | 11374 | 459 | 4 Inter-assay | 1 | 20 | 1865 | 199 | 10.7 Inter-assay | 2 | 20 | 4108 | 346 | 8.4 Inter-assay | 3 | 20 | 11391 | 798 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail