Human Parathyroid Hormone, PTH ELISA Kit

Parathyroid hormone (PTH), parathormone or parathyrin, is secreted by the chief cells of the parathyroid glands as a polypeptide. PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Parathyroid hormone (PTH) has been proved to play a pivotal role in maintaining myocardial contractility as well as effective natriuresis, and possible pathogenic mechanisms contributing to heart failure secondary to hypocalcemia and hypoparathyroidism. With the increased population of preosteoblastic lineages and the osteoblastic activation, Parathyroid hormone (PTH) drives anabolism in bone. Experiments have recently reported that PTH affects bone cells in a dual pathway - mediating osteoblastic (preosteoblastic) activities or osteocytic synthesis of sclerostin. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps.

Concentration | O.D. | Average | Corrected ------------------------------------------ 20000 pg/ml | 2.348 | 2.346 | 2.234 2.343 10000 pg/ml | 1.36 | 1.389 | 1.277 1.417 5000 pg/ml | 0.796 | 0.763 | 0.651 0.729 2500 pg/ml | 0.4 | 0.427 | 0.315 0.453 1250 pg/ml | 0.259 | 0.267 | 0.155 0.275 625 pg/ml | 0.179 | 0.18 | 0.068 0.18 312.5 pg/ml | 0.133 | 0.134 | 0.022 0.134 0 pg/ml | 0.117 | 0.112 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1772 | 96.9 | 5.5 Intra-assay | 2 | 20 | 3691 | 124 | 3.4 Intra-assay | 3 | 20 | 15378 | 350 | 2.3 Inter-assay | 1 | 20 | 1758 | 94.6 | 5.4 Inter-assay | 2 | 20 | 3660 | 199 | 5.5 Inter-assay | 3 | 20 | 15562 | 768 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail