Human Junctional Adhesion Molecule-Like, JAML ELISA Kit

Junctional adhesion molecules (JAMs) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites. JAML (Junctional adhesion molecule-like), also known as AMICA1 (Adhesion molecule interacting with CXADR antigen 1), a protein related to the JAM family, is restricted to leukocytes and promotes their adhesion to endothelial cells. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail involved in activation signaling. Monocytic JAML/AMICA1 plays a critical role in regulating monocyte transendothelial migration (TEM) probably via binding to the endothelial coxsackie and adenovirus receptor (CAR) and other tight junction-associated adhesive molecules. The Expression of JAML/AMICA1 is restricted to the hematopoietic tissues with the exception of liver. JAML may function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, JAML/AMICA1 also enhances myeloid leukemia cell adhesion to endothelial cells.

Concentration | O.D. | Average | Corrected ------------------------------------------ 15000 pg/ml | 3.186 | 3.051 | 2.941 2.915 7500 pg/ml | 1.47 | 1.432 | 1.322 1.394 3750 pg/ml | 0.549 | 0.534 | 0.424 0.519 1875 pg/ml | 0.275 | 0.276 | 0.166 0.276 937.5 pg/ml | 0.188 | 0.177 | 0.067 0.166 468.75 pg/ml | 0.13 | 0.136 | 0.026 0.142 234.375 pg/ml | 0.122 | 0.116 | 0.006 0.109 0 pg/ml | 0.112 | 0.11 | 0 0.108

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1110 | 88.5 | 8 Intra-assay | 2 | 20 | 3171 | 155 | 4.9 Intra-assay | 3 | 20 | 8345 | 288 | 3.4 Inter-assay | 1 | 20 | 1119 | 98.6 | 8.8 Inter-assay | 2 | 20 | 3143 | 217 | 6.9 Inter-assay | 3 | 20 | 8265 | 499 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail