Human Cystatin-F, CST7 ELISA Kit

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 15000 pg/ml | 2.512 | 2.594 | 2.5 2.675 7500 pg/ml | 1.242 | 1.194 | 1.1 1.145 3750 pg/ml | 0.656 | 0.641 | 0.547 0.625 1875 pg/ml | 0.482 | 0.46 | 0.366 0.437 937.5 pg/ml | 0.251 | 0.242 | 0.148 0.232 468.75 pg/ml | 0.152 | 0.146 | 0.052 0.14 234.375 pg/ml | 0.114 | 0.111 | 0.017 0.108 0 pg/ml | 0.097 | 0.094 | 0 0.091

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 723 | 37.3 | 5.2 Intra-assay | 2 | 20 | 2261 | 75.9 | 3.4 Intra-assay | 3 | 20 | 9042 | 244 | 2.7 Inter-assay | 1 | 20 | 721 | 64 | 8.9 Inter-assay | 2 | 20 | 2249 | 230 | 10.2 Inter-assay | 3 | 20 | 9075 | 861 | 9.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail